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Current possibillities of laboratory diagnostics of EBV
Janů, Denisa ; Janďourek, Ondřej (advisor) ; Konečná, Klára (referee)
Charles university Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study programme: Bioanalytical laboratory diagnostics in health care Author: Bc. Denisa Janů Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: Current possibillities of laboratory diagnostics of EBV Background: The aim of this diploma thesis was to get acquainted with the issue of Epstein-Barr virus infection and its laboratory diagnostics. Other aim was to divide the patients into groups according to the serological results, then evaluate the prevalence of EBV and evaluate the incidence of primary EBV infection in the tested group of patients for the years 2019-2022. Methods: Chemiluminescence immunoassay and indirect immunofluorescence were used to determine specific antibodies against EBV. In some cases, the examination was completed by determining the avidity of anti-VCA IgG antibodies and the microblot array method. Real-time PCR was used to detect EBV DNA. Microsoft Excel 2016 was used to process data and to create tables and graphs. Results: For the years 2019-2022, 14,736 patients were tested for the presence of specific antibodies against EBV in the infectious serology and virology laboratory of Vidia-Diagnostika, 263 patients for the presence of heterophile antibodies...
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The possibility of determination of HIV antibodies avidity for early infection detection
Diničová, Michaela ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: The possibility of determination of HIV antibodies avidity for early infection detection The determination of early HIV infection in the Czech Republic is based on a combination of results of laboratory examination, clinical examination, and information from an epidemiological report. The identification of patient in the acute phase of the disease allows to search for targeted contact to reduce further spread of HIV, as patients in the early phase of the disease are the riskiest for transmission of infection. One of the laboratory markers of the acute phase of infection is low avidity of antibodies, which expresses the strength of bonds between antigen and antibody. The determination of avidity is based on fact that avidity in recent infections is low because antibodies do not bind tightly enough to antigen and avidity increases when the immune response matures. Quantitatively, it is expressed by the avidity index, which determines what part of the antibodies (%) will remain bound in complex with the antigen after incubation with chaotropic agent, in our case with guanidine...
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The possibility of determination of HIV antibodies avidity for early infection detection
Diničová, Michaela ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: The possibility of determination of HIV antibodies avidity for early infection detection The determination of early HIV infection in the Czech Republic is based on a combination of results of laboratory examination, clinical examination, and information from an epidemiological report. The identification of patient in the acute phase of the disease allows to search for targeted contact to reduce further spread of HIV, as patients in the early phase of the disease are the riskiest for transmission of infection. One of the laboratory markers of the acute phase of infection is low avidity of antibodies, which expresses the strength of bonds between antigen and antibody. The determination of avidity is based on fact that avidity in recent infections is low because antibodies do not bind tightly enough to antigen and avidity increases when the immune response matures. Quantitatively, it is expressed by the avidity index, which determines what part of the antibodies (%) will remain bound in complex with the antigen after incubation with chaotropic agent, in our case with guanidine...
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